Related Experiment Video
Updated: Jan 27, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Extraction, isolation and structural characterization of a novel polysaccharide from Cyclocarya paliurus
Xiaojiang Wang1, Xiaoliang Zhao1, Youjing Lv2
1Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China.
Abstract:
A water soluble polysaccharide CP-III was extracted and purified from Cyclocarya paliurus. CP-III is identified as a novel pectin-like polysaccharide with molecular weight (Mw) of 72.7 kDa. The structural features of CP-III were characterized by methylation and nuclear magnetic resonance (NMR) spectroscopy. Its depolymerized fragments were analyzed by hydrophilic interaction chromatography-Fourier transform mass spectrometry (HILIC-FTMS). The main chain of CP-III is composed of →4)GalAp(α1 → and →2)Rhap(α1 → 4)GalAp(α1→, repeatedly. The residue of →4)Galp(β1 → and →5)Araf(α1 → alternately exists on the O-4 of partial →2)Rhap(α1 → residues as side chains. On the O-3 of sectional →5)Araf(α1 → residues is a secondary branch assembled by →3)Araf(α1→. Moreover, on the non-reducing terminus of →4)Galp(β1 → occasionally have an →5)Araf(α1 → chain. Surprisingly, a sub-branch constructed by →6)Hexp(β1 → with a galacturonate or methyl galacturonate exists on the O-3 of certain →4)Galp(β1 → residues in the non-reducing terminus. In addition, a terminal Xyl is located on the O-3 of fractional →4)GalAp(β1 → residue. The highly branched polysaccharide CP-III with high water solubility can be used as food supplement and medicinal carrier in the future.
Related Concept Videos
Biosynthesis of Polysaccharides
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the...
Structures of Solids
Structural Isomerism
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
Structure of Lipids
Viral Structure

