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Updated: Jan 27, 2026

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Published on: July 6, 2022
Mitochondrial function in immature bovine oocytes is improved by an increase of cellular cyclic AMP
Shu Hashimoto1, Masaya Yamanaka2, Takayuki Yamochi3
1Graduate School of Medicine, Osaka City University, Osaka, 545-8585, Japan. hashimoto@ivfnamba.com.
Abstract:
Although in vitro maturation (IVM) of oocytes is important for assisted reproduction, the rate of development of embryos from IVM oocytes is lower than from their in vivo counterparts. It has been shown that an artificial increase of intracellular cAMP before culture significantly improves oocyte developmental competence in cattle and mice. Here, we revealed that forskolin and 3-isobutyl-1-methylxanthine treatment of prophase-stage oocytes induced the expression of genes required for glycolysis, fatty acid degradation, and the mitochondrial electron transport system and improved mitochondrial functions and ATP levels in oocytes without involving nuclear maturation. We propose the existence of a comprehensive energy-supply system in oocytes under follicle-stimulating hormone stimulation as a potential explanation of how oocytes acquire developmental competence.
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