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Updated: Jan 27, 2026

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
Published on: July 2, 2016
Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil
Antonio Charlys da Costa1,2, Elcio Leal3, Danielle Gill4,5
1Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, no 470, São Paulo, São Paulo, 05403-000, Brazil. charlysbr@yahoo.com.br.
Abstract:
The nearly complete genome sequences of two Cucumis melo endornavirus (CmEV) strains were obtained using deep sequencing while investigating fecal samples for the presence of gastroenteritis viruses. The Brazilian CmEV BRA/TO-23 (aa positions 116-5027) and BRA/TO-74 (aa positions 26-5057) strains were nearly identical to the reference CmEV CL-01 (USA) and SJ1 (South Korea) strains, showing 97% and 98% of nucleotide and amino acid identity, respectively. Endornaviruses are not known to be associated with human disease and their presence may simply reflect recent dietary consumption. Metagenomic analyses offered an opportunity to identify for the first time in Brazil a newly described endornavirus species.
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