Enhanced Immunoassay Using a Rotating Paper Platform for Quantitative Determination of Low Abundance Protein

Abootaleb Sedighi1, Ulrich J Krull1

  • 1Department of Chemical and Physical Sciences , University of Toronto Mississauga , 3359 Mississauga Road , Mississauga , Ontario L5L 1C6 , Canada.

Analytical Chemistry
|March 28, 2019
PubMed

Related Concept Videos

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems01:22

Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems

Body:Bioavailability is a critical pharmacological concept that measures the extent and rate at which an active drug ingredient or therapeutic moiety enters the systemic circulation, remaining unchanged. It's a pivotal factor in determining a drug's efficacy and safety.The Biopharmaceutics Classification System (BCS) plays an essential role in drug development by categorizing drugs into four classes based on their solubility and permeability. This classification aids in understanding drug...
200
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
642
Kinematic Equations for Rotation01:30

Kinematic Equations for Rotation

In mechanics, when one observes a rigid body in rotational motion with constant angular acceleration, it is possible to establish equations for its rotational kinematics. This process resembles how linear kinematics are dealt with in simpler motion studies.
For instance, imagine a point A on a rigid body engaged in circular motion. The translational velocity of this particular point can be calculated by taking the time derivatives of the displacement equation, which essentially measures the...
788
Rotation of Asymmetric Top01:11

Rotation of Asymmetric Top

By definition, a spherically symmetric body has the same moment of inertia about any axis passing through its center of mass. This situation changes if there is no spherical symmetry. Since most rigid bodies are not spherically symmetric, these require special treatment.
The relationship between the angular momentum of any rigid body and its angular velocity, both of which are vectors, involves the moment of inertia. The moment of inertia is a scalar quantity only for spherically symmetric...
1.5K
Rotational Motion about a Fixed Axis01:26

Rotational Motion about a Fixed Axis

A rigid body's rotation around a fixed axis makes every point within it trace a circular path around a specific line or point. The term given to this type of spinning is defined by the angular position, symbolized by the angle θ. This angle is gauged from a static reference line to the revolving object. From this angular position, any variation is referred to as angular displacement, denoted by dθ. The extent of this displacement can be calculated in degrees, radians, or...
1.3K
Rotation with Constant Angular Acceleration - I01:37

Rotation with Constant Angular Acceleration - I

If angular acceleration is constant, then we can simplify equations of rotational kinematics, similar to the equations of linear kinematics. This simplified set of equations can be used to describe many applications in physics and engineering where the angular acceleration of a system is constant.
Using our intuition, we can begin to see how rotational quantities such as angular displacement, angular velocity, angular acceleration, and time are related to one another. For example, if a flywheel...
8.2K