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LncRNA CASC11 improves atherosclerosis by downregulating IL-9 and regulating vascular smooth muscle cell apoptosis

Kaiyu Tao1, Zhanglong Hu1, Yigong Zhang1

  • 1a Department of cardiovascular surgery , Second Affiliated Hospital of Zhejiang University School of Medicine , Hangzhou City , Zhejiang Province , PR China.

Bioscience, Biotechnology, and Biochemistry
|March 28, 2019
PubMed

Insights

Long non-coding RNA CASC11, previously an oncogene, is downregulated in atherosclerosis. It may protect against this cardiovascular disease by reducing IL-9 levels and regulating vascular smooth muscle cell (VSMC) proliferation and apoptosis.

Area of Science:

  • Cardiovascular Biology
  • Molecular Oncology
  • Epigenetics

Background:

  • Long non-coding RNA CASC11 is an established oncogene in various cancers.
  • The role of LncRNA CASC11 in the pathogenesis of atherosclerosis remains unexplored.
  • Interleukin-9 (IL-9) is implicated in inflammatory processes relevant to cardiovascular disease.

Purpose of the Study:

  • To investigate the role of LncRNA CASC11 in atherosclerosis.
  • To determine the relationship between CASC11 and IL-9 in atherosclerosis patients.
  • To elucidate the functional impact of CASC11 on vascular smooth muscle cells (VSMCs).

Main Methods:

  • Quantitative analysis of CASC11 and IL-9 plasma levels in atherosclerosis patients and healthy controls.
  • Correlation analysis between CASC11 and IL-9 levels.
  • In vitro experiments involving IL-9 treatment and CASC11 overexpression in VSMCs.
  • Assessment of VSMC proliferation and apoptosis.

Main Results:

  • Plasma levels of CASC11 were downregulated, while IL-9 was upregulated in atherosclerosis patients compared to controls.
  • Altered CASC11 and IL-9 levels effectively distinguished patients from controls.
  • A significant inverse correlation between CASC11 and IL-9 was observed in atherosclerosis patients.
  • CASC11 overexpression downregulated IL-9 expression and inhibited VSMC proliferation while promoting apoptosis.

Conclusions:

  • LncRNA CASC11 may play a protective role in atherosclerosis.
  • CASC11 appears to exert its protective effects by downregulating IL-9.
  • CASC11 influences VSMC behavior, impacting proliferation and apoptosis, which are critical in atherosclerosis development.
Keywords:
AtherosclerosisIL-9lncRNA CASC11

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