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Glycoengineering of Antibodies for Modulating Functions
Lai-Xi Wang1, Xin Tong1, Chao Li1
1Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA; email: wang518@umd.edu , xtong1@umd.edu , chaoli@umd.edu , johnpgiddens@gmail.com , kevintiezhengli@gmail.com.
Annual Review of Biochemistry
|March 28, 2019
Summary
Glycoengineering enables precise control over antibody N-glycans, enhancing their therapeutic potential. These advanced techniques allow for the production of homogeneous antibody glycoforms for improved drug efficacy and targeted delivery.
Area of Science:
- Biochemistry
- Immunology
- Glycoscience
Background:
- Antibodies, essential immunoglobulins, are glycoproteins with conserved N-linked oligosaccharides (N-glycans) at the Fc domain.
- Antibody N-glycans significantly influence biological functions and therapeutic effectiveness.
- Heterogeneous antibody glycoforms pose challenges for purification and functional studies.
Purpose of the Study:
- To review major glycoengineering approaches for antibody modification.
- To highlight recent advances in producing defined and site-specifically modified antibodies.
- To focus on glycoengineering for enhanced therapeutic applications, including antibody-drug conjugates.
Main Methods:
- Glycoengineering via manipulation of the glycan biosynthetic pathway.
- In vitro chemoenzymatic glycan remodeling for antibody modification.
- Site-specific antibody-drug conjugation strategies enabled by glycoengineering.
Main Results:
- Glycoengineering facilitates the production of homogeneous antibody glycoforms.
- Site-specific modifications enhance antibody functionality and therapeutic efficacy.
- Advanced glycoengineering methods enable precise control over antibody glycosylation.
Conclusions:
- Glycoengineering is crucial for developing well-defined antibodies with improved therapeutic properties.
- Advances in glycoengineering offer powerful tools for antibody-based drug development.
- Future research will likely focus on further refining these techniques for clinical applications.
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