Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

2.5K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
2.5K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.1K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.1K
Quantitative Aspects of Drug-Receptor Interaction01:30

Quantitative Aspects of Drug-Receptor Interaction

1.7K
The receptor occupancy theory connects a drug's response to the number of occupied receptors. With higher drug concentrations, more receptors are occupied, leading to increased responses. The formation of drug-receptor complexes involves association and dissociation rates, which reach equilibrium when the forward and backward reactions are equal. The equilibrium association constant (Ka) and its inverse, the equilibrium dissociation constant (Kd), indicate drug affinity. Higher Ka and lower...
1.7K
Non-nuclear Inheritance01:29

Non-nuclear Inheritance

23.1K
Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm⁠—such as chloroplasts and mitochondria⁠—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
23.1K
Nuclear Stability03:18

Nuclear Stability

23.1K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
23.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The 2R, 3R isomer of 2, 3-butanediol prevents weight gain and decreases plasma glucose concentration of mice fed a fat-enriched diet.

Frontiers in physiology·2026
Same author

Deciphering <i>cis</i>-regulatory elements using REgulamentary.

Bioinformatics advances·2026
Same author

Human MASLD is a diurnal disease driven by multisystem insulin resistance and reduced insulin availability at night.

Cell metabolism·2026
Same author

Clocks on steroids: how glucocorticoid receptors tell cells the time.

The Journal of endocrinology·2026
Same author

Working against the clock: does night shift work drive multisystem disease?

European journal of preventive cardiology·2026
Same author

Glucocorticoid-dependence and independence of the circadian liver transcriptome.

Npj biological timing and sleep·2026
Same journal

A Computational Systems Biology View on the Role of the Menstrual Cycle in Endocrine Health and Disease.

Journal of molecular endocrinology·2026
Same journal

Pancreatic β-cell aging in physiology and diabetes: emerging roles of m6A mRNA methylation.

Journal of molecular endocrinology·2026
Same journal

Neuroendocrine regulation of female fertility: the role of CNS-derived hormones.

Journal of molecular endocrinology·2026
Same journal

Hypothalamic neuropeptides as modulators of neural activity and behaviour.

Journal of molecular endocrinology·2026
Same journal

Massively parallel functional genomic assays in endocrinology: from promise to delivery.

Journal of molecular endocrinology·2026
Same journal

TSH promotes chemerin/CMKLR1-cAMP/ERK-DIO2 signaling in primary rat ependymal cells in vitro.

Journal of molecular endocrinology·2026
See all related articles

Related Experiment Video

Updated: Jan 27, 2026

Author Spotlight: Using the Split Retina Technique for Enhanced Access and Accelerated Experiments
07:53

Author Spotlight: Using the Split Retina Technique for Enhanced Access and Accelerated Experiments

Published on: January 16, 2024

5.5K

An improved method for quantitative ChIP studies of nuclear receptor function.

Ann Louise Hunter1, Natasha Narang1, Matthew Baxter1,2

  • 1Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

Journal of Molecular Endocrinology
|March 28, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a new chromatin immunoprecipitation (ChIP) method. It combines droplet digital PCR and a spike-in control for accurate quantification and normalization of ChIP-DNA, enhancing experimental reliability.

Keywords:
ChIPddPCRdigital PCRnuclear receptorstroubleshooting

More Related Videos

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.7K
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
10:08

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

2.5K

Related Experiment Videos

Last Updated: Jan 27, 2026

Author Spotlight: Using the Split Retina Technique for Enhanced Access and Accelerated Experiments
07:53

Author Spotlight: Using the Split Retina Technique for Enhanced Access and Accelerated Experiments

Published on: January 16, 2024

5.5K
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.7K
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
10:08

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

2.5K

Area of Science:

  • Molecular Biology
  • Genomics
  • Endocrinology

Background:

  • Chromatin immunoprecipitation (ChIP) is crucial for studying nuclear receptor recruitment in endocrine research.
  • ChIP experiments are complex, with multiple steps prone to error and data interpretation challenges.
  • Accurate quantification of protein-bound DNA is essential for reliable ChIP data analysis and troubleshooting.

Purpose of the Study:

  • To develop a robust ChIP strategy for endocrine researchers.
  • To improve the accuracy and reliability of ChIP data quantification and normalization.
  • To provide a method for confident protocol optimization in ChIP experiments.

Main Methods:

  • Integration of droplet digital PCR (ddPCR) for precise quantification of DNA targets.
  • Implementation of an internal spike-in control for effective data normalization.
  • Application of the combined method to chromatin immunoprecipitation (ChIP) assays.

Main Results:

  • The combined ChIP strategy offers absolute and sensitive quantification of protein-bound DNA.
  • The use of a spike-in control enhances data normalization, allowing for reliable comparisons between experimental conditions.
  • The method increases the overall confidence in ChIP assay results and facilitates troubleshooting.

Conclusions:

  • The developed ChIP strategy significantly improves the reliability of endocrine research data.
  • Droplet digital PCR combined with a spike-in control provides a powerful tool for accurate ChIP analysis.
  • This approach empowers researchers to optimize their ChIP protocols with greater confidence and accuracy.