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[miRNA-96-5p inhibits the proliferation and migration of gastric cancer cells by targeting FoxQ1]

X Y Yang1, N Li2, W Y Deng2

  • 1Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China.

Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|March 28, 2019
PubMed

Insights

MicroRNA-96-5p is downregulated in gastric cancer and suppresses tumor growth. Its overexpression inhibits gastric cancer cell proliferation and invasion by targeting FoxQ1, reducing epithelial-mesenchymal transition (EMT).

Area of Science:

  • Molecular Oncology
  • Cancer Biology
  • MicroRNA Research

Background:

  • Gastric cancer is a significant global health concern with complex molecular underpinnings.
  • MicroRNAs play crucial roles in cancer development and progression, acting as potential therapeutic targets.
  • The specific role of microRNA-96-5p (miRNA-96-5p) in gastric cancer pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the functional role of miRNA-96-5p in gastric cancer cell proliferation and invasion.
  • To elucidate the molecular mechanism by which miRNA-96-5p exerts its effects in gastric cancer.
  • To determine the relationship between miRNA-96-5p and forkhead box Q1 (FoxQ1) expression in gastric cancer.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC) were used to assess miRNA-96-5p and FoxQ1 expression in gastric cancer tissues and cell lines.
  • Gastric cancer cells (BGC-823) were transfected with miRNA-96-5p mimics to evaluate its effects on proliferation (CCK-8 assay) and invasion (Transwell assay).
  • Western blot analysis was performed to measure protein levels of FoxQ1, E-cadherin, and vimentin. Dual-luciferase reporter assays confirmed the direct interaction between miRNA-96-5p and FoxQ1.

Main Results:

  • miRNA-96-5p expression was significantly lower in gastric cancer tissues compared to adjacent normal tissues.
  • FoxQ1 expression was significantly higher in gastric cancer tissues and negatively correlated with miRNA-96-5p levels.
  • Overexpression of miRNA-96-5p suppressed gastric cancer cell proliferation and invasion, reduced FoxQ1 protein levels, upregulated E-cadherin, and downregulated vimentin, indicating inhibition of epithelial-mesenchymal transition (EMT).

Conclusions:

  • miRNA-96-5p acts as a tumor suppressor in gastric cancer.
  • miRNA-96-5p inhibits gastric cancer cell proliferation, invasion, and EMT by downregulating the expression of FoxQ1.
  • These findings highlight miRNA-96-5p as a potential therapeutic target for gastric cancer treatment.
Keywords:
Epithelial-mesenchymal transition (EMT)Forkhead box Q1Gastric neoplasmsInvasionMicroRNAProliferation

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