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Harvesting Sperm and Artificial Insemination of Mice
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Harvesting Sperm and Artificial Insemination of Mice

Published on: April 28, 2007

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Sperm matrix metalloproteinase-2 activity increased in pregnant couples treated with intrauterine insemination: a

Shirin Sharifi1, Roohollah Mohseni2, Iraj Amiri3

  • 1a Department of Biology, Basic Sciences Faculty , Islamic Azad University of Hamedan , Hamedan , Iran.

Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology
|March 29, 2019
PubMed

Insights

Higher sperm matrix metalloproteinase-2 (MMP2) activity is linked to increased pregnancy rates in couples undergoing intrauterine insemination (IUI). This suggests MMP2 may be a key factor in IUI success.

Area of Science:

  • Reproductive Biology
  • Biochemistry

Background:

  • Matrix metalloproteinases (MMPs), specifically MMP2 and MMP9, play crucial roles in the reproductive system and fertilization.
  • Previous research indicates MMP2 activity is essential for sperm-zona pellucida fusion and in vitro fertilization (IVF) rates.

Purpose of the Study:

  • To investigate the activity of MMP2 and MMP9 in semen.
  • To determine the association between MMP2 and MMP9 activity and pregnancy rates, semen parameters, and oxidative stress markers in couples undergoing intrauterine insemination (IUI).

Main Methods:

  • Semen specimens were collected from 60 men whose spouses underwent IUI with controlled ovarian stimulation.
  • MMP2 and MMP9 activity, total thiol groups (TTG), total antioxidant capacity (TAC), and lipid peroxidation (LP) were measured.
  • Pregnancy outcomes were recorded for the couples.

Main Results:

  • Sperm MMP2 activity and seminal plasma MMP9 activity were significantly higher in the pregnant group compared to the non-pregnant group (p < .05).
  • Sperm MMP2 activity correlated positively with seminal plasma TTG and TAC.
  • Sperm MMP9 activity showed a positive correlation with TAC and an inverse correlation with LP.
  • Seminal plasma MMP2 activity correlated with sperm viability and TTG.

Conclusions:

  • Sperm MMP2 activity may be a significant predictor of pregnancy rates following IUI.
  • MMP2 activity in sperm could influence IUI outcomes and is an important factor for IUI success.
  • Further research into MMP2's role could enhance clinical strategies for IUI.
Keywords:
Intrauterine inseminationmatrix metalloproteinase-2matrix metalloproteinase-9pregnancysemensperm

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