Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of cardiac operation on platelets.

R Mohr, M Golan, U Martinowitz

    The Journal of Thoracic and Cardiovascular Surgery
    |September 1, 1986
    PubMed
    Summary

    Extracorporeal circulation impacts platelet size and function. Bleeders showed smaller platelets and abnormal aggregation, suggesting a platelet membrane defect, not factor VIII changes, contributing to bleeding risk.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction.

    Science advances·2021
    Same author

    Serum adiponectin levels in patients with diffuse idiopathic skeletal hyperostosis (DISH).

    Clinical rheumatology·2018
    Same author

    Invasive Aspergillosis in Neutropenic Patients with Hematological Disorders.

    Leukemia & lymphoma·2016
    Same author

    Fifteen years of the Family Eating and Activity Habits Questionnaire (FEAHQ): an update and review.

    Pediatric obesity·2013
    Same author

    Mitochondrial VDAC1: function in cell life and death and a target for cancer therapy.

    Current medicinal chemistry·2011
    Same author

    Haemoglobin O in An Arab Family.

    British medical journal·2010

    Area of Science:

    • Cardiovascular Surgery
    • Hematology
    • Platelet Physiology

    Background:

    • Extracorporeal circulation (ECC) is a critical component of cardiac surgery.
    • Understanding its impact on hemostasis, particularly platelet function, is vital for patient safety.
    • Postoperative bleeding remains a significant complication, necessitating research into its underlying causes.

    Purpose of the Study:

    • To investigate the effects of ECC on platelet count and mean platelet volume (MPV).
    • To assess platelet aggregation responses to various agonists post-ECC.
    • To correlate platelet parameters and aggregation patterns with postoperative bleeding in patients undergoing ECC.

    Main Methods:

    • Prospective study of 65 patients undergoing procedures requiring ECC.

    Related Experiment Videos

  • Measurement of platelet count and MPV pre- and post-operatively.
  • Platelet aggregation studies using adenosine diphosphate, collagen, and ristocetin in 20 patients.
  • Analysis of bleeding times and correlation with platelet function tests.

    • Main Results:

    • Platelet counts decreased post-ECC in both bleeding and non-bleeding groups, with no significant difference.
    • Patients experiencing bleeding (bleeders) exhibited significantly lower MPV and lower percentage of platelet volume compared to non-bleeders.
    • All 20 patients showed abnormal postoperative platelet aggregation, with distinct patterns observed for ristocetin response, correlating with prolonged bleeding times in severe cases.

    Conclusions:

    • ECC affects platelet size and function, with smaller platelet volume and impaired aggregation being characteristic of patients with postoperative bleeding.
    • The observed von Willebrand-like reaction to ristocetin suggests a platelet membrane defect, rather than alterations in Factor VIII, as a potential cause of bleeding.
    • MPV and platelet aggregation assays may serve as valuable indicators for predicting bleeding risk in patients undergoing ECC.