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Updated: Jan 27, 2026

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
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Screening for NTRK-rearranged Tumors Using Immunohistochemistry: Comparison of 2 Different pan-TRK Clones in Melanoma

Amélie Bourhis1, Geoffroy Redoulez1, Isabelle Quintin-Roué1

  • 1Department of Pathology, CHRU Brest, Brest.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|March 29, 2019
PubMed

Insights

This study compared two pan-TRK immunohistochemistry (IHC) clones for screening NTRK-rearranged melanomas. Neither clone reliably identified NTRK rearrangements, highlighting the need for improved diagnostic methods for targeted cancer therapies.

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Cancer Research

Background:

  • NTRK-rearranged tumors are treatable with anti-TRK targeted therapies.
  • Pan-TRK immunohistochemistry (IHC) is a primary screening method for these alterations.
  • Accurate screening is crucial for effective patient treatment.

Purpose of the Study:

  • To compare the diagnostic performance of two pan-TRK IHC clones (A7H6R and EPR17341).
  • To evaluate their efficacy in identifying NTRK rearrangements in advanced melanoma samples.

Main Methods:

  • Comparative analysis of two pan-TRK IHC clones (A7H6R, EPR17341) on 300 melanoma samples.
  • Confirmation of IHC-positive cases using NTRK1, NTRK2, and NTRK3 fluorescent in situ hybridization (FISH).
  • Assessment of staining in non-tumor tissues and use of a positive control.

Main Results:

  • A7H6R IHC identified 4 positive samples, while EPR17341 identified none.
  • None of the 4 samples positive by A7H6R IHC showed NTRK rearrangement via FISH.
  • Non-specific staining was observed in kidney tissue; both clones stained the positive control.

Conclusions:

  • Current pan-TRK IHC clones may have limitations in accurately screening for NTRK rearrangements in melanomas.
  • Further studies with larger cohorts of confirmed NTRK-rearranged tumors are needed.
  • Improved diagnostic strategies are required for selecting patients for TRK-targeted therapies.

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