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Published on: October 9, 2014
A novel coumarin-based fluorescent sensor for Ca2+ and sequential detection of F- and its live cell imaging
Kun Yao1, Yongxin Chang1, Bai Li1
1Institute of Functional Organic Molecular Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.
Abstract:
A novel fluorescent sensor CPM for relay detecting Ca2+ and F- based on coumarin has conveniently synthesized and characterized. The sensor CPM showed highly fluorescence enhancement to Ca2+ over other metal ions, and the CPM-Ca2+ complex could selectively recognize F- among other anions. The limits of detection for Ca2+ and F- were 5.81 × 10-7 M and 4.28 × 10-7 M in aqueous solution (DMF/HEPES buffer 1:1 v/v, 10 mM, pH = 7.2), respectively. Their sensing mode had been testified by Job's plots, UV-vis titration, 1H NMR titrations, ESI-mass, fluorescence and DFT calculations. The fluorescence imaging indicated that CPM was cell-permeable and could be used to effectively detect Ca2+ and F- within living cells.
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