Related Experiment Video
Updated: Jan 27, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Rapid Procedure for Biochemical Characterization and Serological Confirmation of Suspect Salmonella Isolates
J S Bailey1, N A Cox1, J E Thomson1
1United States Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, Athens, Georgia 30613.
Abstract:
Fifty-two freshly processed broiler carcasses were examined for the presence of Salmonella by using a rinse method. Three selective plating media (bismuth sulfite, brilliant green sulfa and Hektoen enteric) were compared. After 24 h of incubation, typical colonies were picked from each selective plate. An 8-h procedure to biochemically characterize (Micro ID) and serologically (poly O and poly H) confirm Salmonella was then compared with a conventional procedure. Suspect Salmonella isolates were correctly classified from 63% of the carcasses with both the 8-h and conventional procedures. Of the 244 isolates confirmed to be Salmonella by conventional testing, 236 (97%) were also confirmed by the 8-h procedure. Brilliant green sulfa and Hektoen enteric agar were superior to bismuth sulfite agar for Salmonella recovery. The 8-h procedure required less incubation time (8 h vs. 48 h) after colony formation, less incubation space, and less media preparation and cleanup than the conventional procedure.
More Related Videos
09:10Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 18, 2010
10:57Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Related Concept Videos
Confirmation Biases
Blind Procedures
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail...
Handwashing III: During the Procedure and Post-Procedure Steps
Rapidly Varying Flow
Tracheostomy: Procedure and Tubes
Tracheostomy tubes can be made of semiflexible plastic (polyurethane or silicone), rigid plastic, or metal, and they come in...