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Updated: Jan 27, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
Overnight Enumeration of Vibrio parahaemolyticus In Seafood by Hydrophobic Grid Membrane Filtration
Phyllis Entis1, Peter Boleszczuk1
1QA Laboratories Limited, 135 The West Mall, Toronto, Ontario, Canada, M9C 1C2.
Abstract:
A method was developed for direct enumeration of Vibrio parahaemolyticus in foods by hydrophobic grid membrane filter. The method consisted of a 4-5 h resuscitation step to recover injured cells, followed by overnight incubation at 42°C on V. parahaemolyticus Sucrose (VPS) agar, a new selective and differential medium. The confirmation rate of typical colonies on VPS agar was greater than 98%. The new method produced significantly higher counts of V. parahaemolyticus than the FDA method (P<0.01) when tested with chill-, freeze- or heat-stressed samples, and was equivalent to the FDA method (P>0.05) for recovery of osmotically stressed V. parahaemolyticus .
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