In-depth proteomic analysis of Boleophthalmus pectinirostris skin mucus

Fish skin mucus serves as the first line of defence against pathogens and external stressors. The mudskipper Boleophthalmus pectinirostris inhabits intertidal mudflats containing abundant and diverse microbial populations; thus, the skin and mucus of B. pectinirostris are very important for immune defence. However, the molecules involved in the immune response and mucus secretion in the skin of this fish are poorly understood. To explore the proteomic profile of the skin mucus and understand the molecular mechanisms underlying B. pectinirostris adaption to amphibious environments, the microstructure of B. pectinirostris skin was analysed, and a series of histochemical procedures were employed for mucous glycoprotein localization and characterization. In addition, the antibacterial activity of B. pectinirostris skin mucus was studied, and the transcriptome of the skin and in-depth proteome of the mucus were determined. These studies revealed the hierarchical structure of B. pectinirostris skin and different types of glycoproteins (GPs) in the dermal bulge (DB) of the B. pectinirostris skin epidermis. The mucus has a broad antimicrobial spectrum and significant effects on the bacterial morphology. Furthermore, 93,914 unigenes were sequenced from B. pectinirostris skin tissue, and a total of 559 proteins were identified from B. pectinirostris skin mucus. SIGNIFICANCE.