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Absence of the long noncoding RNA H19 results in aberrant ovarian STAR and progesterone production

Yong Chen1, Jing Wang2, Yanhong Fan3

  • 1Department of Human Anatomy and Histology, and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, 350122, PR China; Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, CT, USA.

Molecular and Cellular Endocrinology
|March 30, 2019
PubMed

Insights

Noncoding RNAs H19 and let-7 regulate steroid hormone production. Loss of H19 disrupts ovarian steroidogenic acute regulatory protein (STAR) and alters progesterone levels in mice.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Reproductive Biology

Background:

  • Steroidogenesis, crucial for reproduction, is regulated by the steroidogenic acute regulatory protein (STAR).
  • STAR expression is tissue-specific and tightly controlled.
  • Previous in vitro studies identified H19 and let-7 as posttranscriptional regulators of STAR.

Purpose of the Study:

  • To investigate the in vivo function of H19 and let-7 in regulating STAR.
  • To determine the role of cAMP in this regulatory pathway.
  • To assess the impact of H19 loss on ovarian STAR and progesterone production.

Main Methods:

  • Utilized an H19 knockout (H19KO) mouse model.
  • Investigated STAR regulation in vivo.
  • Measured progesterone levels.

Main Results:

  • Demonstrated that H19-mediated regulation of STAR is functional in vivo and responsive to cAMP.
  • Observed disrupted ovarian STAR expression in H19KO mice.
  • Found altered progesterone production in H19KO mice.

Conclusions:

  • Noncoding RNA H19 plays a significant role in regulating ovarian STAR expression and steroid hormone production in vivo.
  • This regulatory mechanism, mediated by noncoding RNAs, is important for steroidogenesis.
  • Further research into noncoding RNA regulation of STAR is warranted.
Keywords:
H19Long noncoding RNAProgesteroneStARSteroidogenesiscAMPlet-7lncRNA

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