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Updated: Jan 27, 2026

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
A cheminformatics approach to characterize metabolomes in stable-isotope-labeled organisms
Hiroshi Tsugawa1,2, Ryo Nakabayashi3, Tetsuya Mori3
1RIKEN Center for Sustainable Resource Science, Yokohama, Japan. hiroshi.tsugawa@riken.jp.
Abstract:
We report a computational approach (implemented in MS-DIAL 3.0; http://prime.psc.riken.jp/) for metabolite structure characterization using fully 13C-labeled and non-labeled plants and LC-MS/MS. Our approach facilitates carbon number determination and metabolite classification for unknown molecules. Applying our method to 31 tissues from 12 plant species, we assigned 1,092 structures and 344 formulae to 3,604 carbon-determined metabolite ions, 69 of which were found to represent structures currently not listed in metabolome databases.
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