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Published on: August 31, 2014
Multiple HIV-1 Subtypes Were Found Circulating in Suqian District of Jiangsu Province, China
Bing Pei1, Lei Jia2, Zhenjiang Zhang1
11 Department of Clinical Laboratory, Suqian First Hospital, Suqian, Jiangsu, China.
Insights
This study reveals a complex HIV-1 epidemic in Suqian, China, with multiple circulating subtypes, including the dominant CRF01_AE strain. High levels of drug resistance were also detected, necessitating enhanced prevention efforts.
Area of Science:
- Virology
- Epidemiology
- Public Health
Background:
- Jiangsu province faces a severe human immunodeficiency virus type 1 (HIV-1) epidemic.
- Suqian, located in northern Jiangsu, lacks detailed HIV epidemic information.
- Understanding the local HIV-1 epidemiology is crucial for targeted interventions.
Purpose of the Study:
- To characterize the HIV-1 epidemic details in Suqian.
- To determine the circulating HIV-1 subtypes and their lineages.
- To assess the prevalence of drug resistance in the Suqian HIV-1 population.
Main Methods:
- Collected 196 plasma samples from treated HIV-1-positive individuals.
- Extracted viral RNA and amplified partial HIV-1 pol genes.
- Sequenced 84 partial pol genes for subtyping and resistance analysis.
Main Results:
- Multiple HIV-1 subtypes are circulating in Suqian.
- CRF01_AE is the dominant subtype, comprising multiple previously identified Chinese lineages.
- A high level of HIV drug resistance was observed in the study population.
Conclusions:
- The HIV-1 epidemic in Suqian is complex and diverse.
- The dominance of CRF01_AE and high drug resistance underscore the need for tailored prevention and intervention strategies.
- Further public health measures are essential for controlling the HIV-1 epidemic in this region.
Abstract:
Jiangsu province has severe HIV-1 epidemic in China. Suqian which is located in north of the province has limited HIV epidemic information. Therefore, this study aimed to characterize the epidemic details in the area. A total of 196 plasma samples were collected from treated HIV-1-positive cases and viral RNA was extracted. Then HIV partial pol genes (nucleotide 2147-3462 by using HXB2 as calibrator) were amplified and sequenced. Finally, 84 partial pol genes were successfully obtained. The subtyping results indicate that multiple HIV-1 subtypes are circulating in Suqian district. Thereinto, CRF01_AE has been the dominant stains here and belonged to multiple lineages of CRF01_AE identified in China previously. Moreover, there is a high level of HIV drug resistance. All these results suggest HIV-1 epidemic in Suqian is rather complex and more measures must be performed for prevention and intervention in the area.
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