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Updated: Jan 27, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
Published on: January 7, 2019
Real time light intensity based carbon dioxide feeding for high cell-density microalgae cultivation and biodiesel
Venkateswara R Naira1, Debasish Das1, Soumen K Maiti1
1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Abstract:
Outdoor high cell-density microalgae cultivation is highly challenging due to unavailability of appropriate CO2 feeding strategy under diurnal sunlight intensities. Hence, a novel real time light based CO2 feeding strategy was firstly developed under diurnal simulated sunlight (LED) to test on Chlorella sp. in a 10 L scale bubble column photobioreactor. The strategy yielded a biomass titer of 5.12 g L-1 under simulated sunlight, far higher than existing biomass-density and pH-control based CO2 feeding strategies. In outdoor culturing, the proposed feeding strategy yielded high biomass titers of 6.8 and 9.0 g L-1 in growth-phase of two-stage and single-stage lipid induction studies respectively with same biomass productivity of 0.8 g L-1 day-1. Subsequently, two-stage lipid induction strategy of 6.8 g L-1 titer yielded biodiesel productivity of 120 g L-1 day-1, whereas single-stage strategy of 9.0 g L-1 titer was unable to induce lipid. Moreover, specific light availability affects the lipid production.
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