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Comparison of Selective Plating Media for Enumeration of Bacillus cereus in Foods

S M Harmon1, D A Kautter1, F D McCLURE1

  • 1Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

Journal of Food Protection
|March 31, 2019
PubMed

Insights

This study compared three agars for enumerating Bacillus cereus in raw sprouts. Mannitol-egg yolk-polymyxin (MYP) agar allowed easier differentiation of B. cereus, reducing confirmatory tests.

Area of Science:

  • Food Microbiology
  • Bacterial Enumeration Techniques

Background:

  • Bacillus cereus is a common foodborne pathogen found in various foods, including raw sprouts.
  • Accurate enumeration of B. cereus is crucial for food safety assessments.
  • Different selective agars are used for isolating and identifying B. cereus, but their efficacy can vary.

Purpose of the Study:

  • To compare the effectiveness of three different agar media for the enumeration of Bacillus cereus in raw mung bean and wheat sprouts.
  • To evaluate the ease of differentiation and need for confirmatory tests when using each medium.

Main Methods:

  • Raw mung bean and wheat sprouts were artificially inoculated with ten different strains of Bacillus cereus.
  • Enumeration of B. cereus was performed using three agar media: mannitol-egg yolk-polymyxin (MYP), polymyxin pyruvate-egg yolk-mannitol-bromthymol blue, and trypticase-soy-polymyxin blood.
  • Recovery rates for B. cereus on each medium were statistically compared.

Main Results:

  • No significant differences were observed in the recovery rates of Bacillus cereus across the three tested agar media.
  • Mannitol-egg yolk-polymyxin (MYP) agar demonstrated superior performance in differentiating B. cereus from other microorganisms present in the sprout samples.
  • The use of MYP agar significantly reduced the number of confirmatory tests required for accurate identification.

Conclusions:

  • All three tested media are suitable for the enumeration of Bacillus cereus in raw sprouts based on recovery rates.
  • Mannitol-egg yolk-polymyxin (MYP) agar is recommended for routine analysis due to its enhanced ability to differentiate B. cereus, thereby improving laboratory efficiency.

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