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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
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Design and optimisation of Photochrome Aptamer Switch Assay (PHASA)
Yubin Zhou1, Yuanyuan Wu2, Oleksandr Pokholenko2
1School of Materials Science & Engineering, College of Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore; Department of Physiology and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117456, Singapore.
Analytica Chimica Acta
|March 31, 2019
Summary
This study introduces the Photochrome Aptamer Switch Assay (PHASA), a novel fluorescent biosensing platform. New analytical methods are proposed to optimize biosensor sensitivity and limit of detection for improved performance.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Materials Science
Background:
- The Photochrome Aptamer Switch Assay (PHASA) is a novel fluorescent biosensing platform.
- PHASA leverages aptamer binding and stilbene photoisomerization for reversible biosensing.
- Biosensor performance is influenced by binding affinity, aptamer concentration, and stilbene structure.
Purpose of the Study:
- To propose and discuss new analytical methods for calculating the limit of detection (LOD) in PHASA.
- To enable optimization of physicochemical parameters for stilbene-ligand conjugates.
- To evaluate the general sensing performance of PHASA based on structure-activity relationships.
Main Methods:
- Development of analytical methods for LOD calculation in PHASA.
- Analysis of inter-dependent relationships between sensitivity and analyte detection range.
- Utilizing known aptamer binding constants for PHASA parameterization.
Main Results:
- Proposed analytical methods provide a framework for optimizing PHASA performance.
- Understanding structure-activity relationships of stilbene-ligand conjugates is crucial for sensitivity.
- The methods facilitate the prediction and enhancement of biosensor sensitivity.
Conclusions:
- New analytical methods enhance the optimization and evaluation of PHASA biosensors.
- Physicochemical parameter optimization is key to improving biosensor sensitivity and LOD.
- PHASA offers a versatile platform for various aptamer-based sensing applications.
Keywords:
AptamerFluorescence assayLimit of detectionPhotochrome aptamer switch assay (PHASA)PhotoisomerisationStilbeneMore Related Videos
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