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Tankyrase1 antisense oligodeoxynucleotides suppress the proliferation, migration and invasion through Hippo/YAP

Yichi Zhou1, Qi Jin1, Wei Xiao1

  • 1Department of Orthopaedics, CR & WISCO General Hospital, Wuhan, 430000, PR China.

Pathology, Research and Practice
|March 31, 2019
PubMed

Insights

Tankyrase1 (TANK1) is upregulated in osteosarcoma. Inhibiting TANK1 with TANK1-ASODN suppressed cancer cell growth, migration, and invasion, offering a potential therapeutic strategy for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Osteosarcoma is a primary bone cancer with high metastatic potential and poor patient outcomes.
  • Understanding the molecular drivers of osteosarcoma progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of tankyrase1 (TANK1) in osteosarcoma development.
  • To explore the therapeutic potential of targeting TANK1 in osteosarcoma cells.

Main Methods:

  • Osteosarcoma MG-63 cells were transfected with tankyrase1 antisense oligodeoxynucleotides (TANK1-ASODN).
  • Cell proliferation, migration, and invasion were assessed using CCK-8, immunofluorescence, wound healing, and Transwell assays.
  • mRNA and protein levels of TANK1 were quantified using RT-qPCR and Western blot.
  • The Hippo/YAP signaling pathway was analyzed.

Main Results:

  • TANK1 expression was found to be upregulated in osteosarcoma cells.
  • TANK1-ASODN transfection significantly inhibited MG-63 cell proliferation, migration, and invasion.
  • Epithelial-mesenchymal transition (EMT) was suppressed in TANK1-ASODN treated cells.
  • TANK1-ASODN modulated the Hippo/YAP signaling pathway.

Conclusions:

  • TANK1 plays a significant role in promoting osteosarcoma progression.
  • Inhibition of TANK1 via TANK1-ASODN demonstrates therapeutic promise by suppressing key cancer hallmarks.
  • The Hippo/YAP pathway is implicated in the mechanism by which TANK1 influences osteosarcoma.
Keywords:
Antisense oligodeoxynucleotidesEpithelial-mesenchymal transitionHippoOsteosarcomaTankyrase1YAP

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