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Functional analysis of lymphoid cells from mouse bone marrow in vitro
Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
|February 1, 1978
Summary
Mouse bone marrow cells show distinct responses to mitogens like phytohaemagglutinin and lipopolysaccharide. Researchers identified specific cell surface markers to differentiate pre-T cells with antibody-dependent cell-mediated cytotoxicity activity from pre-B cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Bone marrow (BM) cells are crucial for immune responses.
- Understanding progenitor cell populations is key to immunology.
Purpose of the Study:
- To characterize the in vitro responses of mouse BM cells to mitogens.
- To identify cell surface markers for differentiating pre-T and pre-B cells.
Main Methods:
- In vitro stimulation of BM cells with phytohaemagglutinin (PHA), concanavalin A (Con A), and lipopolysaccharide (LPS).
- Fractionation of BM cells based on cell surface characteristics, including ADCC activity, Fc receptors (FcR), membrane immunoglobulin (Ig), and C3 receptors (C3R).
- Comparison of fractionation results with established methods using Ig anti-Ig columns.
Main Results:
- BM cells responded to PHA, Con A, and LPS in vitro.
- A significant population of BM cells exhibited antibody-dependent cell-mediated cytotoxicity (ADCC) activity.
- Pre-T cells were associated with ADCC activity and Fc receptors (FcR) and/or membrane Ig.
- Pre-B cells responded to LPS, lacked ADCC activity, and were negative for Ig, FcR, and C3 receptors (C3R).
Conclusions:
- Specific cell surface markers can distinguish between pre-T and pre-B cell populations in mouse bone marrow.
- Pre-T cells possess ADCC activity and Fc receptors, while pre-B cells respond to LPS and lack these markers.