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Related Experiment Videos

Cellodextrin utilization and beta-glucosidase production by Bacteroides polypragmatus.

C R MacKenzie, G B Patel

    Archives of Microbiology
    |June 1, 1986
    PubMed
    Summary

    Bacteroides polypragmatus ferments glucose and cellobiose into ethanol. This anaerobic bacterium produces a stable intracellular beta-glucosidase enzyme, crucial for cellodextrin breakdown.

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    Area of Science:

    • Microbiology
    • Enzymology
    • Biochemistry

    Background:

    • Bacteroides polypragmatus is an anaerobic bacterium.
    • Understanding its metabolic pathways is key to its biotechnological applications.

    Purpose of the Study:

    • To investigate the fermentation capabilities of Bacteroides polypragmatus.
    • To characterize the beta-glucosidase enzyme produced by this bacterium.

    Main Methods:

    • Culturing Bacteroides polypragmatus under anaerobic conditions.
    • Analyzing fermentation end-products using gas chromatography.
    • Enzyme purification and characterization, including optimal pH, Km values, and stability assays.

    Main Results:

    • Simultaneous fermentation of glucose and cellobiose yielding ethanol as a major product.
    • Utilization of higher cellodextrins.
    • Characterization of an intracellular beta-glucosidase (pI 4.2, MW ~100,000 Da) with optimal activity at pH 7.
    • Enzyme exhibited stability at 55°C for 4 hours with glycerol and was stable during long-term storage.

    Conclusions:

    • Bacteroides polypragmatus efficiently ferments simple and complex carbohydrates to ethanol.
    • The characterized beta-glucosidase is a robust intracellular enzyme with potential for industrial applications.

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