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Albumin does not inhibit endothelium-dependent relaxation
Biochemical and Biophysical Research Communications
|August 14, 1986
Summary
Fatty acid-free bovine serum albumin enhances endothelium-dependent vasodilation in rat aortic rings. However, plasma and serum abolished this vasodilating effect, indicating albumin
Area of Science:
- Pharmacology
- Physiology
Background:
- Endothelium-derived relaxing factors mediate vasodilation.
- The role of albumin in modulating vascular responses is not fully understood.
Purpose of the Study:
- To investigate the effect of fatty acid-free bovine serum albumin on endothelium-dependent vasodilation.
- To compare the effects of albumin, plasma, and serum on vascular responses.
Main Methods:
- Rat aortic rings with intact endothelium were used.
- Vasodilation was measured in response to various agonists (acetylcholine, carbachol, ATP, ADP, ionophore) in the presence of different albumin concentrations, plasma, or serum.
Main Results:
- Fatty acid-free bovine serum albumin (BSA) significantly enhanced endothelium-dependent vasodilation.
- The maximal enhancement was observed at 5% BSA concentration.
- BSA had no effect on aortic rings without endothelium.
- Both plasma and serum completely abolished the vasodilating effects of the tested agonists.
Conclusions:
- Fatty acid-free BSA potentiates endothelium-dependent vasodilation.
- Plasma and serum interfere with or inhibit endothelium-dependent vasodilation, potentially due to factors other than albumin.