Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

"Redox lipidomics technology: Looking for a needle in a haystack".

Yulia Y Tyurina1, Vladimir A Tyurin1, Tamil Anthonymuthu2

  • 1Department of Environmental and Occupational Health, Pittsburgh, PA, USA.

Chemistry and Physics of Lipids
|April 1, 2019
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TranDep: a transcriptomics atlas of depression.

Molecular psychiatry·2026
Same author

15-LOX-catalytic bias towards ether-(alkenyl)-ETE-PEs oxidation bestows selectivity of PRO-ferroptotic cell death signaling.

Nature communications·2026
Same author

Expert opinion on burning questions in cancer myeloid cell biology.

Journal for immunotherapy of cancer·2026
Same author

Harnessing myeloid cell plasticity for cancer therapy.

Nature cancer·2026
Same author

Comparative analysis of milk and brain fatty acids reveals human-specific signatures in brain development.

Communications biology·2026
Same author

Corrigendum to: Mitochondrial bioenergetics and cardiolipin remodeling abnormalities in mitochondrial trifunctional protein deficiency.

JCI insight·2026

Redox lipidomics reveals peroxidized lipids are key signaling molecules in physiological processes like cell death and immune responses. This field uses advanced mass spectrometry techniques to decode these complex lipid communication pathways.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Aerobic life relies on metabolic oxidation and oxygenated signaling compounds, including polyunsaturated fatty acids (PUFA).
  • Lipidomics, particularly redox lipidomics, has emerged due to advances in liquid-chromatography-mass spectrometry (LC-MS) for characterizing oxygenated PUFA-containing phospholipids.
  • While lipid peroxidation was linked to injury, enzymatic phospholipid oxygenation is now recognized in essential physiological pathways.

Purpose of the Study:

  • To review contemporary protocols for identifying and quantifying peroxidized (phospho)lipids.
  • To discuss the application of LC-MS in analyzing peroxidized lipids in apoptosis, ferroptosis, phagocytosis, and cancer.
  • To explore novel MS imaging techniques for spatial distribution analysis and innovative data interpretation methods.
Keywords:
Lipid mediatorsMass spectrometryOxidatively truncated lipidsPeroxidized phospholipidsRedox lipidomics

Related Experiment Videos

Main Methods:

  • Liquid-chromatography-mass spectrometry (LC-MS) for identification and quantification of peroxidized (phospho)lipids.
  • Mass spectrometry (MS) imaging, including high-resolution techniques, for spatial distribution analysis.
  • Innovative data interpretation approaches, such as audio-representation analysis.

Main Results:

  • LC-MS enables detailed characterization of peroxidized cardiolipins and phosphatidylethanolamines in apoptosis and ferroptosis.
  • Peroxidized phosphatidylserines play a role in phagocytotic signaling.
  • Peroxidized tri-acylglycerides are involved in immunosuppressive signaling in cancer.

Conclusions:

  • Redox lipidomics provides a rich communication language through the analysis of peroxidized (phospho)lipids.
  • Enzymatically catalyzed phospholipid oxygenation is crucial for various physiological processes.
  • Advanced analytical techniques are essential for understanding the complex roles of peroxidized lipids.