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Updated: Jan 27, 2026
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
Metastatic prostate cancer cells are highly sensitive to 3-bromopyruvic acid
Monika Pichla1, Jolanta Sroka2, Natalia Pienkowska1
1Department of Analytical Biochemistry, Faculty of Biology and Agriculture, University of Rzeszów, Zelwerowicza Street 4, 35-601 Rzeszów, Poland.
Aims:
3-Bromopyruvate (3-BP), an alkylating agent and a glycolytic inhibitor, is a promising anticancer agent, which can be efficient also against multidrug-resistant cancer cells. The aim of this study was to examine how 3-BP affects the survival and mobility of rat (MAT-LyLu and AT-2) and human (DU-145 and PC-3) metastatic prostate cancer cell lines.
Main Methods:
Cytotoxicity was estimated with Neutral Red. Cell mobility was analyzed by time-lapse microscopic monitoring of trajectories of individual cells at 5-min intervals for 6h. ATP was estimated with luciferin/luciferase and glutathione (GSH) with o-phthalaldehyde. Actin cytoskeleton was visualized with phalloidin conjugated with Atto-488.
Key Findings:
All metastatic prostate cell lines studied were very sensitive to 3-BP (IC50 of 4-26μM). 3-Bromopyruvate drastically reduced cell movement even at concentrations of 5-10μM after 1h treatment. This compound depleted also cellular ATP and GSH, and disrupted actin cytoskeleton.
Significance:
The data obtained suggest that 3-BP can potentially be useful for treatment of metastatic prostate cancer and, especially, be efficient in limiting metastasis.
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