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MicroRNA-101-3p suppresses proliferation and migration in hepatocellular carcinoma by targeting the HGF/c-Met pathway

Yang Liu1, Juan Tan1, Shuangyan Ou2

  • 1Department of Pathology, Infectious Diseases Institute, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.

Investigational New Drugs
|April 1, 2019
PubMed

Insights

MicroRNA-101 (miR-101) suppresses hepatocellular carcinoma (HCC) by targeting the HGF/c-Met pathway. Upregulating miR-101 inhibits tumor growth and cell migration, offering a potential therapeutic strategy for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) are crucial in tumor development.
  • The hepatocyte growth factor (HGF)/c-Met pathway promotes cancer cell proliferation and migration.
  • The role of miR-101 in hepatocellular carcinoma (HCC) requires further definition.

Purpose of the Study:

  • To investigate the interaction between HGF and miRNAs in HCC.
  • To define the role of miR-101 in HCC progression.
  • To explore the therapeutic potential of miR-101 in HCC.

Main Methods:

  • Bioinformatics analysis to identify miRNA targets.
  • Luciferase reporter assays to confirm direct targeting.
  • In vitro cell culture experiments to assess proliferation and migration.
  • In vivo studies using animal models to evaluate tumor suppression.

Main Results:

  • Hepatocyte growth factor (HGF) was upregulated, while microRNA-101-3p (miR-101) was significantly downregulated in HCC tumor tissues.
  • HGF was identified as a direct target of miR-101.
  • miR-101 inhibited HCC cell migration and proliferation by targeting the HGF/c-Met axis.
  • Overexpression of miR-101 suppressed tumor growth in vivo.

Conclusions:

  • miR-101 acts as a tumor suppressor in HCC by negatively regulating the HGF/c-Met pathway.
  • miR-101 demonstrates potential as a targeted therapeutic agent for HCC treatment.
  • miRNAs represent a promising class of drugs for clinical application in HCC therapy.
Keywords:
HGFHepatocellular carcinomac-METmiR-101

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