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Updated: Jan 27, 2026
09:15
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Published on: November 21, 2017
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Value of DSC in Characterization and Optimization of Protein Stability
Katherine Bowers1, Natalia Markova2
1Principal Scientist/Group Leader Analytical and Formulation Development, FUJIFILM Diosynth Biotechnologies U.S.A., Inc., Morrisville, NC, USA. katherine.bowers@fujifilm.com.
Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2019
Abstract:
Differential scanning calorimetry (DSC) is a well-established technique for biomolecular stability studies. The technique is based on forced thermal denaturation of biomolecules in solution. Here we describe the use of DSC for characterization and optimization of stability of two proteins, a protein kinase and a mAb protein.
Keywords:
Buffer optimizationDSCDifferential scanning calorimetryMultiple stability metricsPre-formulationProtein stabilityMore Related Videos
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