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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
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Proteomic study of bioactive peptides from tempe
Badrut Tamam1, Dahrul Syah2, Maggy Thenawidjaja Suhartono2
1Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Darmaga, Bogor, West Java 16680, Indonesia; Department of Nutrition, Polytechnic of Health, Denpasar, Bali 80237, Indonesia.
Journal of Bioscience and Bioengineering
|April 2, 2019
Summary
This study identified bioactive peptides in Indonesian tempeh, revealing that higher sanitation levels correlate with a greater abundance of these beneficial compounds, including those with antihypertensive and antidiabetic properties.
Area of Science:
- Food Science
- Biochemistry
- Nutraceuticals
Background:
- Tempeh, a traditional Indonesian fermented soybean, is widely produced by small industries.
- Research on tempeh's bioactive peptides remains limited.
- Sanitation practices in tempeh production may influence bioactive peptide content.
Purpose of the Study:
- To investigate and characterize bioactive peptides in tempeh from producers with varying sanitation levels.
- To identify specific peptides and assess their potential bioactivities.
- To determine the relationship between sanitation and bioactive peptide diversity.
Main Methods:
- Water extraction and ultrafiltration (<3 kDa) of tempeh samples.
- Peptide separation using gel filtration chromatography and RP-HPLC.
- Identification via LC-MS, spectral prediction (FindPept), and biofunctionality confirmation (BIOPEP).
Main Results:
- Identified unique and common bioactive peptides across three tempeh producers.
- Val-His and Ala-Leu-Glu-Pro peptides were consistently found.
- Higher sanitation levels correlated with increased bioactive peptide content (58% good, 43% moderate, 35% poor sanitation).
Conclusions:
- Tempeh contains bioactive peptides with potential antihypertensive, antidiabetic, antioxidative, and antitumor properties.
- Sanitation significantly impacts the quantity and diversity of bioactive peptides in tempeh.
- This highlights tempeh as a promising source of functional food ingredients.
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