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Related Experiment Videos

Relationship between circulating plasminogen activators and tumor development in mice.

M Colombi, L Rebessi, M Boiocchi

    Cancer Research
    |November 1, 1986
    PubMed
    Summary

    Murine tumor cells release urokinase-type plasminogen activator (u-PA), distinct from tissue-type plasminogen activator (t-PA) in mouse plasma. Increased plasma u-PA correlates with tumor growth, while its absence may facilitate metastasis.

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    Clinical genetics·2017

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Oncology

    Background:

    • Plasminogen activators (PAs) are crucial in extracellular matrix remodeling.
    • Distinguishing between tissue-type (t-PA) and urokinase-type (u-PA) PAs is vital for understanding their roles in physiological and pathological processes.
    • Murine tumor models offer a platform to study PA dynamics in cancer development.

    Purpose of the Study:

    • To characterize plasminogen activators in BALB/c mice plasma and murine tumor cell lines.
    • To investigate the relationship between tumor development, metastasis, and the levels of t-PA and u-PA.
    • To elucidate the distinct roles of t-PA and u-PA in tumor progression.

    Main Methods:

    • Electrophoretic-zymographic techniques were employed to analyze PA activities.

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  • Murine tumor cell lines (B77-3T3, SR-BALB, AA6) and BALB/c mouse plasma were used for characterization.
  • Tumorigenic and irradiated cells, as well as lymphocytes, were injected into mice to assess PA responses.

    • Main Results:

    • BALB/c mouse plasma contains a main PA activity identified as murine t-PA (MW 88,000, pI 6.3).
    • Murine tumor cells release a distinct PA activity identified as murine u-PA (MW 44,500, pI 9.2).
    • Tumor development correlated with increased plasma t-PA and u-PA, with u-PA detectable prior to visible tumor formation. Metastatic cells showed no detectable plasma u-PA.

    Conclusions:

    • Plasma u-PA levels are linked to tumor development, independent of metastatic potential.
    • The absence of plasma u-PA may promote metastasis formation.
    • PA characterization provides insights into tumor biology and metastatic processes.