Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice

Hippocampal inhibitory interneurons exhibit a large diversity of dendritic Ca²⁺ mechanisms that are involved in the induction of Hebbian and anti-Hebbian synaptic plasticity. High resolution imaging techniques allowed examining somatic Ca²⁺ signals and, accordingly, the recruitment of hippocampal interneurons in awake behaving animals. However, little is still known about dendritic Ca²⁺ activity in interneurons during different behavioral states. Here, we used two-photon Ca²⁺ imaging in mouse hippocampal CA1 interneurons to reveal Ca²⁺ signal patterns in interneuron dendrites during animal locomotion and immobility. Despite overall variability in dendritic Ca²⁺ transients (CaTs) across different cells and dendritic branches, we report consistent behavior state-dependent organization of Ca²⁺ signaling in interneurons. As such, spreading regenerative CaTs dominated in dendrites during locomotion, whereas both spreading and localized Ca²⁺ signals were seen during immobility. Thus, these data indicate that while animal locomotion is associated with widespread Ca²⁺ elevations in interneuron dendrites that may reflect regenerative activity, local CaTs that may be related to synaptic activity become apparent during animal quiet state.