A human T-T-cell hybridoma-derived lymphocyte chemoattractant factor

Area of Science:

• Immunology
• Cell Biology

Background:

• Lymphokines play a critical role in regulating T-lymphocyte motility.
• Developing strategies to obtain these lymphokines is essential for understanding immune responses.

Purpose of the Study:

• To develop human T-T hybridomas for producing lymphokines that modulate T-lymphocyte motility.
• To characterize the physicochemical properties of the chemoattractant produced by these hybridomas.

Main Methods:

• Fusion of mitogen-stimulated human T lymphocytes with CEM lymphoma cells.
• Assessment of chemoattractant activity using a modified Boyden chamber assay.
• Physicochemical characterization including molecular sieve chromatography, anion-exchange chromatography, isoelectric focusing, and SDS-PAGE.

Main Results:

• Hybridoma 41B2 supernatant significantly enhanced lymphocyte migration.
• The chemoattractant activity was associated with a molecular weight of 14,000 (monomeric form).
• The factor exhibited an isoelectric point of 9.0-9.2 and was sensitive to trypsin and neuraminidase but heat-stable at 56°C.

Conclusions:

• Human T-T-cell hybridomas constitutively produce a lymphocyte chemoattractant.
• The identified chemoattractant is physicochemically identical to human lymphokine, lymphocyte chemoattractant factor.
• This finding provides a valuable tool for studying T-lymphocyte migration and immune regulation.