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Updated: Jan 27, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

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BZW2 gene knockdown induces cell growth inhibition, G1 arrest and apoptosis in muscle-invasive bladder cancers: A

Haifeng Gao1, Guanghai Yu1, Xian Zhang2

  • 1Department of Urology Surgery, Dalian Municipal Central Hospital, Dalian, China.

Journal of Cellular and Molecular Medicine
|April 2, 2019
PubMed

Insights

Basic leucine zipper and W2 domains 2 (BZW2) promotes muscle-invasive bladder cancer (MIBC) growth. Inhibiting BZW2 suppressed tumor cell proliferation and induced apoptosis, suggesting BZW2 as a potential therapeutic target for bladder cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Bladder cancer is a prevalent global malignancy.
  • The role of basic leucine zipper and W2 domains 2 (BZW2) in tumor progression is known, but its specific function in muscle-invasive bladder cancer (MIBC) is unclear.

Purpose of the Study:

  • To investigate the expression and function of BZW2 in human MIBC.
  • To explore the molecular mechanisms by which BZW2 influences MIBC.

Main Methods:

  • Analysis of BZW2 expression in MIBC tissues versus adjacent non-tumour tissues.
  • In vitro studies using shRNA to knockdown BZW2 in bladder cancer cell lines (5637 and T24).
  • In vivo studies using mice xenograft models.
  • Biochemical microarray analysis to identify affected signaling pathways.

Main Results:

  • BZW2 expression was significantly higher in MIBC tissues compared to non-tumour tissues.
  • BZW2 knockdown inhibited cell proliferation and G1/S cell cycle progression in vitro.
  • BZW2 knockdown induced apoptosis in bladder cancer cells.
  • In vivo studies confirmed the anti-proliferative effect of BZW2 knockdown.

Conclusions:

  • BZW2 plays an oncogenic role in muscle-invasive bladder cancer.
  • BZW2 is a potential therapeutic target for MIBC diagnosis and gene therapy.
Keywords:
BZW2microarray pathway analysismuscle-invasive bladder cancers (MIBCs)xenograft model

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