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An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
Published on: September 20, 2022
Identification of Narciclasine as an in Vitro Anti-Inflammatory Component of Cyrtanthus contractus by
Lucie Rárová1, Bhekumthetho Ncube2, Johannes Van Staden2
1Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science , Palacký University , Šlechtitelů 27 , CZ-783 71 Olomouc , Czech Republic.
Abstract:
In this study, an extract from the bulbs of Cyrtanthus contractus showed strong anti-inflammatory activity in vitro. The extract was partially separated into 14 fractions and analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry metabolomics, and the correlation coefficients were calculated between biological activities and metabolite levels. As a result, the top-scoring metabolite narciclasine (1) is proposed as the active principle of C. contractus. This was confirmed by comparing the biological effect of crude extract with that of an authentic standard.
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