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Updated: Jan 27, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Comparison of Aerobic and Anaerobic Incubation Conditions for Optimal Recovery of Salmonella
J S Bailey1, J O Reagan1, N A Cox1
1U.S. Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, P.O. Box 5677, Athens, Georgia 30613 and Department of Food Science, University of Georgia, Athens, Georgia 30602.
Abstract:
Effects of aerobic and anaerobic incubation of selenite-cystine and TT enrichment broth incubated at 37 and 34°C on the growth of Salmonella were determined. Pure cultures of four serotypes of Salmonella were enumerated at 0, 4, 8 and 24 h of incubation and no significant differences related to incubation conditions were found. The effect of microflora other than Salmonella , in pure and mixed cultures and from chicken and feed samples, on the recovery rate of Salmonella after incubation in enrichment media was evaluated and no significant effects were found; however, aerobic incubation usually gave higher Salmonella counts. Greater recovery of Salmonella was obtained by incubating selective plating media aerobically rather than anaerobically. Aerobic incubation of liquid enrichment media and differential plating media is therefore recommended for optimal recovery of Salmonella .
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