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Identification of Enterobacteriaceae in Foods with the AutoMicrobic System.
J S Bailey1, N A Cox1, J E Thomson1
1United States Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, P.O. Box 5677, Athens, Georgia 30613 and Kansas State University, Department of Animal Science and Industry, Manhattan, Kansas 66506.
Journal of Food Protection
|April 3, 2019
Summary
The AutoMicrobic System (AMS) accurately identified Enterobacteriaceae from food sources. This microbial identification method demonstrated high accuracy for both stock cultures and fresh isolates, including Salmonella.
Area of Science:
- Microbiology
- Food Safety
- Clinical Diagnostics
Background:
- Accurate identification of Enterobacteriaceae is crucial for food safety.
- Reliable microbial identification systems are needed for routine laboratory diagnostics.
Purpose of the Study:
- To evaluate the species identification accuracy of the AutoMicrobic System (AMS) for Enterobacteriaceae.
- To compare AMS performance against established identification systems (Micro-ID and API).
Main Methods:
- Tested 136 stock cultures and 163 fresh isolates of Enterobacteriaceae from various food products.
- Utilized the AutoMicrobic System (AMS) for species identification.
- Concurrently used Micro-ID and API systems for comparative analysis.
Main Results:
- AMS achieved 99.3% accuracy for stock cultures (135/136).
- AMS demonstrated 98.2% accuracy for fresh isolates (160/163).
- All 74 Salmonella cultures were correctly identified by AMS.
Conclusions:
- The AutoMicrobic System (AMS) is a highly accurate tool for identifying Enterobacteriaceae species in food samples.
- AMS provides reliable results comparable to other established methods.
- The system is effective for identifying Salmonella, a critical foodborne pathogen.
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