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Effect of Injection Speed on Oocyte Deformation in ICSI
Amir M Hajiyavand1, Mozafar Saadat2, Alessandro Abena3
1Department of Mechanical Engineering, School of Engineering, University of Birmingham, Birmingham B15 2TT, UK. A.Hajiyavand@bham.ac.uk.
Micromachines
|April 3, 2019
Summary
Optimizing injection speed in Intracytoplasmic Sperm Injection (ICSI) is crucial for reducing oocyte deformation and improving infertility treatment success. This study found an optimal speed range to minimize cell damage and ensure successful embryo development.
Area of Science:
- Reproductive Biology
- Biomedical Engineering
- Developmental Biology
Background:
- Oocyte deformation during Intracytoplasmic Sperm Injection (ICSI) can cause cell damage and treatment failure.
- Injection speed is a critical factor influencing oocyte deformation.
Purpose of the Study:
- To investigate the effect of injection speed on zebrafish oocyte deformation.
- To develop analytical and finite element (FE) models to understand the relationship between injection speed, force, and deformation.
- To determine an optimal injection speed range for minimizing oocyte damage during ICSI.
Main Methods:
- Experimental setup designed to study zebrafish embryo deformation at different injection speeds.
- Development of an analytical model linking injection force, deformation, and speed.
- Development and validation of a finite element (FE) model to analyze deformation and stress fields.
- Validation of the FE model against experimental data.
Main Results:
- Increasing injection speed decreased oocyte deformation.
- Higher speeds resulted in increased injection force and force fluctuations, leading to higher vibration.
- An optimal injection speed range was identified to balance deformation and force.
Conclusions:
- The study identified an optimal injection speed range for ICSI to minimize oocyte deformation and damage.
- Validated FE models can predict force-deformation variations, aiding future research on injection parameters.
- Optimizing injection speed is key to improving ICSI success rates in infertility treatments.
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