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Updated: Jan 27, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Published on: July 23, 2017
Entomopathogenic nematodes in pharmaceutical formulations for Rhipicephalus microplus (Acari: Ixodidae) control: In
Alessandra Ésther de Mendonça1, Raiana Gonçalves Moreira2, Maria da Penha Henriques do Amaral3
1Laboratório de Estudos em Pragas e Parasitos - LEPP, do Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Outeiro São João Batista, s/n°, Espaço Multidisciplinar, Campus Valonguinho, Niterói, RJ, CEP. 24020-141, Brazil; Programa de Pós-Graduação em Ciências e Biotecnologia, Universidade Federal Fluminense, Outeiro São João Batista, s/n°, Espaço Multidisciplinar, Campus Valonguinho, Niterói, CEP. 24020-141, RJ, Brazil; Laboratório de Parasitologia da Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, Juiz de Fora, MG, CEP: 36038-330, Brazil.
Abstract:
This work aimed to investigate candidate biological control agents against the cattle tick Rhipicephalus microplus by studying three entomopathogenic nematodes (EPN) isolates from two species, Heterorhabditis bacteriophora (isolates LPP30 and HP88) and Heterorhabditis baujardi (isolate LPP7). The study comprised the pharmacotechnical development of four test formulations, and their biological evaluation, in three stages: 1) selection of the fittest EPN isolate at 16 ± 1 °C after 120 h of incorporation in the formulations; 2) determination of the effects of temperature and incubation time on the survival of the selected isolate in formulations; 3) evaluation of the in vitro efficacy (infectivity) of the selected EPN formulations and their components against R. microplus females after 72 h exposure. H. baujardi LPP7 presented the highest survival rates in 0.1% carboxymethyl cellulose sodium (CMC) formulations (100% at 25 °C, up to 10 days after incorporation). At the highest tested temperature (34.2 °C), 54% of LPP7 survived for up to 72 h in CMC formulations. The infectivity of LPP7-containing formulation against R. microplus was 99%, reducing oviposition, egg production index and larval hatching. Considering the pressing need for safer acaricide products, incorporation of EPNs in pharmaceutical formulations is presented as a promising approach for the biological control of cattle tick infestations, potentially reducing environmental impact and resistance development associated with chemical acaricides.
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