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Updated: Jan 27, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
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Luteolin attenuates high glucose-induced podocyte injury via suppressing NLRP3 inflammasome pathway

Qian Yu1, Minda Zhang2, Lifen Qian1

  • 1Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, PR China.

Life Sciences
|April 3, 2019
PubMed
Abstract

Insights

Luteolin protects against diabetic nephropathy by inhibiting podocyte injury and NLRP3 inflammasome activation. This suggests luteolin

Area of Science:

  • Nephrology
  • Pharmacology
  • Molecular Biology

Background:

  • Diabetic nephropathy is a significant complication of diabetes, linked to inflammation.
  • Luteolin shows potential in managing diabetic complications, but its mechanism in nephropathy is unclear.

Purpose of the Study:

  • To investigate the therapeutic effects of luteolin on diabetic nephropathy.
  • To elucidate the underlying mechanism of luteolin's action, focusing on podocyte injury and NLRP3 inflammasome activation.

Main Methods:

  • Utilized western blot, Real-time PCR, immunofluorescence, and flow cytometry to assess podocyte injury and NLRP3 inflammasome activation.
  • Measured reactive oxygen species (ROS) and malondialdehyde (MDA) levels.
  • Investigated the role of NLRP3 inflammasome in luteolin's anti-apoptotic effects via siNLRP3 transfection.

Main Results:

  • Luteolin protected podocytes from high glucose-induced apoptosis and mitochondrial dysfunction.
  • Luteolin significantly reduced NLRP3 inflammasome assembly and interleukin-1β (IL-1β) secretion.
  • Inhibition of NLRP3 inflammasome abolished luteolin's anti-apoptotic effects, highlighting its central role.

Conclusions:

  • Luteolin demonstrates protective effects against podocyte injury in diabetic nephropathy.
  • Luteolin's therapeutic potential is mediated through the inhibition of NLRP3 inflammasome activation.
  • Luteolin represents a promising therapeutic agent for treating diabetic nephropathy.
Keywords:
ApoptosisDiabetic nephropathyLuteolinNLRP3 inflammasomePodocyteROS

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