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Related Concept Videos

Polymers: Defining Molecular Weight01:01

Polymers: Defining Molecular Weight

3.8K
Unlike small molecules with definite molecular weights, polymers are a mixture of individual polymer chains of varying lengths, each with a unique molecular weight.  So, the molecular weight of a polymer is expressed as an average value based on the average size of the polymer chains. The two most common forms of averages used for polymers are the number average molecular weight and weight average molecular weight.
The number average molecular weight (Mn) is the summation of the number...
3.8K
Polymers: Molecular Weight Distribution01:10

Polymers: Molecular Weight Distribution

4.7K
For any given polymer, the weight average molecular weight (Mw) is higher than, if not equal to, the number average molecular weight (Mn). The only situation in which the weight average molecular weight and the number average molecular weight are equal is when a polymer consists only of chains with equal molecular weight. However, this never happens in a synthetic polymer, since it is difficult to control the polymerization process up to a molecular level with accuracy to a hundred percent.
4.7K
Molecular Weight of Step-Growth Polymers01:08

Molecular Weight of Step-Growth Polymers

2.7K
Step growth polymerization involves bi or multifunctional monomers. Bifunctional monomers react to form linear step growth polymers, whereas multifunctional monomers react to form non-linear or branched polymers.
As the step-growth polymerization involves step-wise condensation of monomers, the molecular weight also builds up eventually. Consequently, high molecular weight polymers are obtained at the late stages of the polymerization, where 99% of monomers have been consumed.
The extent of the...
2.7K
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

1.8K
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
1.8K
DNA Isolation01:34

DNA Isolation

199.2K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
199.2K
DNA Isolation01:24

DNA Isolation

44.9K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
44.9K

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Related Experiment Video

Updated: Jan 27, 2026

Isolation of Genomic DNA from Mouse Tails
07:26

Isolation of Genomic DNA from Mouse Tails

Published on: July 29, 2007

30.0K

Isolation of High-Molecular-Weight DNA from Mouse Tail Tips.

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy

    Cold Spring Harbor Protocols
    |April 3, 2019
    PubMed
    Summary

    Mouse tail tips yield DNA samples through Proteinase K digestion and extraction. These DNA preparations are effective for Southern blotting and polymerase chain reaction (PCR) applications.

    Area of Science:

    • Molecular Biology
    • Genetics

    Background:

    • Efficient DNA extraction is crucial for various downstream genetic analyses.
    • Mouse models are widely used in biomedical research, necessitating reliable sample preparation methods.

    Purpose of the Study:

    • To describe a straightforward method for preparing DNA from mouse tail tips.
    • To confirm the suitability of the extracted DNA for common molecular biology techniques.

    Main Methods:

    • DNA isolation from mouse tail tip tissue.
    • Proteinase K digestion for sample lysis.
    • Subsequent DNA extraction and purification.

    Main Results:

    • Successfully obtained DNA suitable for molecular analysis.
    • Demonstrated DNA quality adequate for Southern blotting.

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    Extraction of High Molecular Weight DNA from Microbial Mats
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    Extraction of High Molecular Weight DNA from Microbial Mats

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    Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

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    Related Experiment Videos

    Last Updated: Jan 27, 2026

    Isolation of Genomic DNA from Mouse Tails
    07:26

    Isolation of Genomic DNA from Mouse Tails

    Published on: July 29, 2007

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    Extraction of High Molecular Weight DNA from Microbial Mats
    09:30

    Extraction of High Molecular Weight DNA from Microbial Mats

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    Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
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  • Confirmed DNA suitability for polymerase chain reaction (PCR).

    • Conclusions:

    • Mouse tail tip DNA preparation using Proteinase K digestion is a reliable method.
    • The extracted DNA is versatile and applicable to Southern blotting and PCR.
    • This protocol provides a foundational step for genetic studies using mouse models.