Isolation of High-Molecular-Weight DNA from Mouse Tail Tips

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Updated: Jan 27, 2026

Isolation of Genomic DNA from Mouse Tails
07:26

Isolation of Genomic DNA from Mouse Tails

Published on: July 29, 2007

30.0K

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy

    Cold Spring Harbor Protocols
    |April 3, 2019
    PubMed

    Insights

    Mouse tail tips yield DNA samples through Proteinase K digestion and extraction. These DNA preparations are effective for Southern blotting and polymerase chain reaction (PCR) applications.

    Area of Science:

    • Molecular Biology
    • Genetics

    Background:

    • Efficient DNA extraction is crucial for various downstream genetic analyses.
    • Mouse models are widely used in biomedical research, necessitating reliable sample preparation methods.

    Purpose of the Study:

    • To describe a straightforward method for preparing DNA from mouse tail tips.
    • To confirm the suitability of the extracted DNA for common molecular biology techniques.

    Main Methods:

    • DNA isolation from mouse tail tip tissue.
    • Proteinase K digestion for sample lysis.
    • Subsequent DNA extraction and purification.

    Main Results:

    • Successfully obtained DNA suitable for molecular analysis.
    • Demonstrated DNA quality adequate for Southern blotting.
    • Confirmed DNA suitability for polymerase chain reaction (PCR).

    Conclusions:

    • Mouse tail tip DNA preparation using Proteinase K digestion is a reliable method.
    • The extracted DNA is versatile and applicable to Southern blotting and PCR.
    • This protocol provides a foundational step for genetic studies using mouse models.

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