Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.The gut microbiome in colorectal cancer (CRC) patients shows consistently higher richness and specific metabolic pathways compared to healthy individuals. These findings identify reproducible microbiome biomarkers for potential clinical prognostic tests.
Area Of Science
- Microbiome research
- Oncology
- Metagenomics
Background
- The gut microbiome's role in colorectal cancer (CRC) is under investigation, but biomarker replicability across populations remains a challenge.
- Existing studies often lack consistency in identifying reliable microbial signatures for CRC.
Purpose Of The Study
- To perform a meta-analysis of fecal metagenomes to identify reproducible gut microbiome biomarkers for colorectal cancer (CRC).
- To validate predictive microbiome signatures and explore the relationship between microbiome function and CRC.
- To establish a foundation for clinical prognostic tests based on microbiome data.
Main Methods
- Meta-analysis of five public and two new fecal metagenome datasets (total 969 samples), with validation on two additional cohorts.
- Analysis of microbiome functional potential and specific gene abundance (choline trimethylamine-lyase).
- Development and validation of predictive microbiome signatures for CRC detection.
Main Results
- Colorectal cancer (CRC) patients exhibited significantly higher gut microbiome richness compared to controls.
- Specific metabolic pathways (gluconeogenesis, putrefaction, fermentation) were associated with CRC, while others (stachyose, starch degradation) were linked to controls.
- Predictive microbiome signatures achieved high accuracy (average AUC 0.84) in independent validation cohorts.
- The choline trimethylamine-lyase gene was found to be overabundant in CRC patients, indicating a link to choline metabolism.
Conclusions
- Reproducible gut microbiome biomarkers for colorectal cancer (CRC) have been identified through combined analysis of heterogeneous cohorts.
- Accurate disease-predictive models based on microbiome signatures can be developed.
- These findings support the potential for microbiome-based clinical prognostic tests and mechanistic studies in CRC.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
Overview
In the 1850s and 1860s, Gregor Mendel investigated inheritance by performing monohybrid crosses in pea plants. He crossed two plants that were true-breeding for different traits. Based on his observations, Mendel proposed that organisms inherit two copies of each trait, one from each parent, and that dominant traits can hide recessive traits. These results formed the basis of two fundamental principles in genetics: the Principle of Uniformity and the Law of Segregation.
Monohybrid...