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Updated: Jan 27, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.6K

Elevated expression of microRNA-328-3p suppresses aggressive malignant behaviors via targeting matrix metalloprotease

Ming Zhang1, Jiayuan Zhang1, Quan Zhou1

  • 1Department of Orthopaedics, Huai'an Hospital Affiliated to Xuzhou Medical College and Huai'an Second Hospital, Huai'an, Jiangsu, People's Republic of China, wuque197928@163.com.

Oncotargets and Therapy
|April 3, 2019
PubMed
Abstract

Insights

MicroRNA-328-3p is downregulated in osteosarcoma, suppressing tumor growth and metastasis. Its regulation of MMP16 highlights its potential as a therapeutic target for osteosarcoma progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Previous research suggested microRNA (miR)-328-3p upregulation inhibits metastasis in osteosarcoma.
  • The clinical relevance and specific functions of miR-328-3p in osteosarcoma were previously undetermined.

Purpose of the Study:

  • To investigate the clinical significance and biological roles of miR-328-3p in human osteosarcoma.
  • To elucidate the molecular mechanisms underlying miR-328-3p's function in osteosarcoma progression.

Main Methods:

  • Quantitative real-time PCR to measure miR-328-3p expression in osteosarcoma tissues.
  • Statistical analysis of miR-328-3p expression with clinicopathological features.
  • In vitro assays (Cell Counting Kit-8, scratch-wound healing) to assess miR-328-3p's functional impact.
  • Luciferase reporter assay, western blot, and rescue assays to identify molecular targets and mechanisms.

Main Results:

  • miR-328-3p expression was significantly lower in osteosarcoma tissues compared to non-cancerous bone.
  • Downregulation of miR-328-3p correlated with advanced stage, metastasis, and recurrence.
  • Enforced miR-328-3p expression inhibited osteosarcoma cell proliferation and migration.
  • Matrix metalloprotease 16 (MMP16) was identified as a direct target, and its overexpression partially reversed miR-328-3p's inhibitory effects.

Conclusions:

  • Aberrant miR-328-3p expression is linked to malignant progression in human osteosarcoma.
  • miR-328-3p acts as a tumor suppressor by inhibiting cell proliferation and migration.
  • The tumor-suppressive function of miR-328-3p is partially mediated through the regulation of MMP16.
Keywords:
cell migrationcell proliferationclinicopathological featurematrix metalloprotease 16microRNA-328-3posteosarcoma

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