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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
A new method and insights for estimating phenological events from herbarium specimens
1Department of Biological Science Florida State University 319 Stadium Drive Tallahassee Florida 32306 USA.
Premise Of The Study:
A novel method of estimating phenology of herbarium specimens was developed to facilitate more precise determination of plant phenological responses to explanatory variables (e.g., climate).
Methods And Results:
Simulated specimen data sets were used to compare the precision of phenological models using the new method and two common, alternative methods (flower presence/absence and ≥50% flowers present). The new "estimated phenophase" method was more precise and extracted a greater number of significant species-level relationships; however, this method only slightly outperformed the simple "binary" (e.g., flowers present/absent) method.
Conclusions:
The new method enables estimation of phenological trends with greater precision. However, when time and resources are limited, a presence/absence method may offer comparable results at lower cost. Using a more restrictive approach, such as only including specimens in a certain phenophase, is not advised given the detrimental effect of decreased sample size on resulting models.
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