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Updated: Jan 27, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Integrating herbarium specimen observations into global phenology data systems.
Laura Brenskelle1, Brian J Stucky1, John Deck2
1Florida Museum of Natural History University of Florida Gainesville Florida USA.
The Plant Phenology Ontology now integrates herbarium specimen data, enabling historical plant phenology analysis. This enhances monitoring and forecasting of plant phenological changes over time.
Area of Science:
- Botany
- Ecology
- Bioinformatics
Background:
- The Plant Phenology Ontology (PPO) was established to unify plant phenology observations from global networks.
- Phenology, the study of plant life cycle timing, is crucial for understanding ecological responses to environmental change.
Purpose of the Study:
- To introduce a new release of the PPO and data pipelines supporting the integration of phenology data from herbarium specimens.
- To expand PPO's capacity to include historical and modern phenological data from diverse sources.
Main Methods:
- Modified PPO with terms to link measurements from plant parts (common in herbarium specimens) to whole-plant phenology.
- Developed a proof-of-concept for ingesting annotations from imaged herbarium sheets of *Prunus serotina* (common black cherry).
Main Results:
- Successfully ingested and analyzed phenology data from herbarium specimens.
- Demonstrated a 125-year analysis of flowering time changes for *Prunus serotina*, highlighting the value of integrated data.
Conclusions:
- Conceptual and technical advancements facilitate herbarium data integration into PPO.
- Future expansion could include data from citizen science platforms using photographs.
- Incorporating herbarium data strengthens capabilities for monitoring, modeling, and forecasting plant phenology shifts.
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