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Updated: Jan 26, 2026

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
Published on: May 27, 2022
Sub-ppm level high energy resolution fluorescence detected X-ray absorption spectroscopy of selenium in articular
C Bissardon1, O Proux2, S Bureau3
1ISTerre, Université Grenoble Alpes, P.O. Box 53, 38041 Grenoble, France and Centre of Nanohealth, Swansea University Medical School, Swansea, UK.
Abstract:
The speciation of highly-diluted elements by X-ray absorption spectroscopy in a diverse range of materials is extremely challenging, especially in biological matrices such as articular cartilage. Here we show that using a high energy resolution fluorescence detected X-ray absorption spectroscopy (HERFD-XAS) technique coupled to an array of crystal analyzers, selenium speciation down to 400 ppb (μg kg-1) within articular cartilage can be demonstrated. This is a major advance in the speciation of highly-diluted elements through X-ray absorption spectroscopy and opens new possibilities to study the metabolic role of selenium and other elements in biological samples.
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