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Updated: Jan 26, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
Development of a Method for Recovery of Rotavirus from the Surface of Vegetables
Amin S Badawy1, Charles P Gerba1, Lee M Kelley1
1Departments of Nutrition and Food Science, and Microbiology and Immunology, University of Arizona, Tucson, Arizona 85721.
Abstract:
Rotaviruses cause waterborne disease outbreaks of gastroenteritis from sewage contaminated water, but methods have not been available to assess the potential for transmission of rotavirus by uncooked foods. A method was developed for recovery of rotavirus from the surface of vegetables. The simian rotavirus SA-11, used as a model for the human rotavirus, was adsorbed onto lettuce and the effects of various eluents tested for its recovery. The maximum recovery of rotavirus occurred with a solution of 3% beef extract at pH 8.0 after 5 min of exposure. Longer exposure times did not enhance virus recovery. Recovery of rotavirus and poliovirus type 1 (LSC) averaged 80 and 65%, respectively. Recovery of rotavirus from non-leafy vegetables was lower, averaging 44% for celery, carrot and radish. This method should prove useful for assessing the occurrence and survival of rotavirus on uncooked foods.
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