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Relationship Between Extracellular Neutral Protease Production and Appearance of Bleb-Like Evaginations in
S S Thompson1, Y M Naidu1, J J Pestka1
1Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824-1224.
Abstract:
Pseudomonas fragi is one of several pseudomonads known to produce proteolytic enzymes. During growth of P. fragi in brain heart infusion broth (BHI) at 10°C, the bacterial population increased from 107 to over 1010 CFU/ml after 130 h, with a concurrent increase in pH from 7.4 to 8.5. Maximal extracellular protease activity occurred after 60 to 72 h. Ultrastructural examination of cells grown in BHI showed the presence of bleb-like evaginations of the cell wall. Similar structures were not detected when P. fragi was grown in Koser citrate broth, a medium which was unsuitable for supporting protease production by P. fragi .
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