Related Experiment Video
Updated: Jan 26, 2026

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
Published on: November 18, 2017
Transcriptome analysis for identifying possible causes of post-reproductive death of Sepia esculenta based on brain
Jinyong Zhang1,2,3, Muchun He4, Zilong Xiang5
1Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
Background:
The subpeduncle lobe/olfactory lobe-optic gland axis is called the endocrine regulation center of cephalopods. However, little is known about the mechanism of the subpeduncle lobe/olfactory lobe-optic gland axis regulate the sexual maturation and post-reproductive death of Sepia esculenta Hoyle.
Objectives:
The primary objective of this study was to provide basic information for revealing the mechanism of the subpeduncle lobe/olfactory lobe-optic axis regulating the rapid post-reproductive death of S. esculenta.
Methods:
In this paper, Illumina sequencing based transcriptome analysis was performed on the brain tissue of female S. esculenta in the three key developmental stages: growth stage (BG), spawning stage (BS), and post-reproductive death stage (BA).
Results:
A total of 66.19 Gb Illumina sequencing data were obtained. A comparative analysis of the three stages showed 2609, 3333, and 170 differentially expressed genes (DEGs) in BG-vs-BA, BG-vs-BA, and BS-vs-BA, respectively. The Gene Ontology (GO) enrichment analysis of DEGs revealed that the regulation of cyclin-dependent protein serine/threonine kinase activity, oxidative phosphorylation, and respiratory chain were significantly enriched. The significant enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway identified pathways associated with the regulation of death, such as the mammalian target of rapamycin (mTOR) signaling pathway, AMPK signaling pathway, oxidative phosphorylation, and cell cycle.
Conclusion:
The post-reproductive death of S. esculenta was found to be a complex energy steady-state regulation network system. The mTOR acted as an energy receptor and had a key role in regulating energy homeostasis.
Related Concept Videos
Reproductive Cloning
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
Overview of the Reproductive System
The gonads, or primary reproductive organs, produce gametes and sex hormones. In males, the testes produce spermatozoa and testosterone, which is responsible for developing secondary male sex characteristics, including a deeper voice, larger muscles, facial and body...
Asexual Reproduction
Disorders of the Female Reproductive System
Disorders of the Male Reproductive System
Prostate disorders are another major concern. These conditions can impair urinary flow due to the prostate's location around the urethra....
Overview of Cell Death
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...

